Comparison of the Responses of Human Melanocytes with Different Melanin Contents to Ultraviolet B Irradiation1

Melanin is thought to serve in photoprotection. To investigate this, we have compared the responses of cultured human melanocytes derived from different pigmentary phenotypes (skin types I-VI) to a single irra diation with different doses of I A li light, ranging between 11.7 and 70.1 m.l/rm'. After I \ I! irradiation, heavily pigmented melanocytes had the same percent survival but a greater capacity to resume proliferation than their lightly pigmented counterparts. A significant increase in melanin content was observed in heavily pigmented but not in lightly pigmented melanocytes. Irradiation with 1X1! light blocked melanocytes, regardless of their melanin content, in (!,, and induced the expression of the tumor suppressor p53 protein within 4 h. This induction steadily increased up to 48 h in lightly pigmented melanocytes; however, in heavily pigmented melanocytes, p53 level peaked at 24 h after DVB treatment and declined thereafter. Additionally, DNA from lightly pigmented melanocytes con tained significantly higher numbers of cyclobutane pyrimidine dimers than did DNA from heavily pigmented melanocytes after irradiation with increasing doses of INI! light. We speculate that the prolonged induction of p53 in lightly pigmented melanocytes arrests them in ( ;, for a long time period in order to repair extensive DNA damage. The above described differences might partially explain the increased susceptibility of individ uals with lightly pigmented skin compared to individuals with dark skin to the photodamaging and photocarcinogenic effects of sun exposure.